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The Proteomics Protocols Handbook: Springer Protocols Handbooks

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Published in The Proteomics Protocols Handbook (Springer Protocols Handbooks)

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The Proteomics Protocols Handbook, part of the prestigious Springer Protocols Handbooks series, is an authoritative compendium of cutting-edge techniques for the analysis of proteins. This comprehensive volume provides a comprehensive collection of detailed protocols for the preparation, separation, identification, quantification, and characterization of proteins.

Key Features

Extensive Coverage: Encompasses a wide range of topics in proteomics, from sample preparation and protein extraction to mass spectrometry and data analysis.
Expert Authors: Written by renowned scientists actively engaged in proteomics research, ensuring accuracy and reliability.
Detailed Protocols: Step-by-step instructions with detailed materials lists, troubleshootings, and notes enhance reproducibility.
High-Quality Illustrations: Informative figures and tables illustrate complex concepts and guide users through experimental procedures.
Updated Content: Reflects the latest advances in proteomics technologies and methodologies, providing researchers with the most up-to-date information.

Target Audience

The Proteomics Protocols Handbook is an indispensable resource for:

Proteomics researchers: Graduate students, postdoctoral fellows, and scientists working in academia or industry.
Biochemists and molecular biologists: Seeking to expand their expertise into proteomics.
Clinical and biomedical research scientists: Investigating protein biomarkers and disease mechanisms.
Analytical chemists: Developing and implementing analytical techniques for protein analysis.

Section 1: Sample Preparation

Sample Collection and Preparation
Protein Extraction from Cells and Tissues
Subcellular Fractionation
Protein Precipitation and Concentration

Section 2: Protein Separation

Gel Electrophoresis (1D and 2D)
Isoelectric Focusing
Chromatography (HPLC and LC-MS)
Capillary Electrophoresis

Section 3: Protein Identification

Mass Spectrometry
Peptide Sequencing
De Novo Sequencing
Database Searching and Protein Identification

Section 4: Protein Quantification

Label-Free Quantification
Isobaric Tagging (iTRAQ and TMT)
Absolute Protein Quantification
Protein Arrays

Section 5: Protein Characterization

Protein Structure Analysis
Protein Interactions
Protein Modifications
Proteomics in Biomedical Research

Benefits of Using The Proteomics Protocols Handbook

Accelerated Research: Access to proven protocols expedites experimental design and execution, saving time and resources.
Enhanced Reproducibility: Detailed guidelines minimize error and maximize the reliability of results.
Comprehensive Knowledge Transfer: Provides a comprehensive overview of proteomics techniques, fostering a deep understanding of the field.
Cutting-Edge Methodology: Stay informed with the latest advancements in proteomics, ensuring access to the most innovative technologies.
Reduced Error and Frustration: Troubleshooting sections and notes help identify and resolve potential problems during experimentation.

The Proteomics Protocols Handbook is an essential reference for anyone involved in proteomics research. With its comprehensive coverage, detailed protocols, and expert authorship, this volume provides a valuable guide to the latest techniques and applications in the field. By utilizing this indispensable resource, researchers can enhance the accuracy and efficiency of their proteomics investigations and gain valuable insights into protein function, structure, and interactions.

The Proteomics Protocols Handbook (Springer Protocols Handbooks)

by Adrian Walker

5 out of 5

Language	:	English
File size	:	12029 KB
Text-to-Speech	:	Enabled
Screen Reader	:	Supported
Enhanced typesetting	:	Enabled
Print length	:	1007 pages

Images

[ Proteomics Protocols Handbook Cover Image ]

[ Protein Separation by Gel Electrophoresis ]

[ Mass Spectrometry for Protein Identification ]

[ Label-Free Quantification for Protein Abundance Measurement ]

[ Protein Structure Analysis by X-ray Crystallography ]

A comprehensive compilation of step-by-step protocols for protein sample preparation, separation, identification, quantification, and characterization, supported by expert guidance and illustrations.